1,1-Dichloroethane → (r)-Thiophene-2-sulfonic acid [4-[4-(4-cyclopentyl-piperazin-1-yl)-1,3-dioxo-1,3-dihydro-isoindol-2-yl]-4-(3,4-dimethoxy-phenyl)-butyl]-amide

Participants

Reaction Steps

A 50-mL round bottom flask was charged with Compound 167 (10.0 mg, 0.016 mmol) and dichloroethane (2.3 mL). Triethylamine (2.5 μL, 0.018 mmol) was added followed by cyclopentanone (7.1 μL, 0.06 mmol) and sodium triacetoxyborohydride (4.7 mg, 0.022 mmol). The mixture was stirred at room temperature for 3 h. The mixture was quenched with saturated aqueous sodium bicarbonate (10 mL) and extracted with dichloromethane (50 mL). The organic layer was dried using Na2SO4, filtered through Celite®, and concentrated in vacuo. The crude oil was purified on a on a Gilson HPLC with a reversed phase Kromasil column (10u, 100 Å C18, column length 250×50 mm, gradient 75:25-0:100 H2O:MeCN) to give 5.1 mg of Compound 193 as a yellow solid. 1H NMR (300 MHz, CDCl3) 7.53-7.64 (m, 3H), 7.44 (d, J=7.2 Hz, 1H), 7.14 (d, J=8.2 Hz, 1H), 6.91-7.06 (m, 3H), 6.79 (d, J=8.0 Hz, 1H), 6.56 (s, 1H), 5.13-5.19 (m, 1H), 4.51-4.64 (m, 1H), 3.88 (s, 3H), 3.85 (s, 3H), 3.73-3.83 (m, 2H), 3.42-3.64 (m, 2H), 2.78-3.22 (m, 6H), 2.48-2.61 (m, 1H), 1.88-2.31 (m, 4H), 1.47-1.69 (m, 4H), and 1.27-1.33 (m, 4H); MS (ES+) 653 (M+1).

Provenance & Evidence