Ammonium Hydroxide → HCID 178455963, compound

Participants

Reaction Steps

H-Ile-Lys-Ser-Val-Arg-Ser-Lys-Lys-Thr-Ala-Lys-Trp(CHO)-Asn-OH (3) (460 mg) was treated with 0.15M ammonium hydroxide (200 mg) at room temperature for 40 minutes. The reaction mixture was neutralized with acetic acid (2.5 ml) and concentrated. The concentrate was chromatographed on a Sephadex LH-20 column (3.2×65 cm, 1% acetic acid) for desalting and lyophilized to give crude H-Ile-Lys-Ser-Val-Arg-Ser-Lys-Lys-Thr-Ala-Lys-Trp-Asn-OH (4) (443 mg).

Provenance & Evidence