反应详情

A solution of 2.3 g of N2 -(6, 7-dimethoxy-2-naphthylsulfonyl)-L-arginyl-N-(2-methoxypropyl)glycine ethyl ester in 15 ml of methanol and 5 ml of 2N sodium hydroxide solution was warmed at 40° C and held at that temperature for 10 hours. At the end of this period, the reaction mixture was concentrated and chromatographed on 200 ml of Daiaion® SK 102 ion exchange resin (200-300 mesh, H+ form, manufactured by Mitsubishi Chemical Industries Limited) packed in water, washed with ethanol-water (1:4) and eluted with ethanol-water-NH4OH (10:9:1). The main fraction was evaporated to dryness and washed with ether to give 1.8 g of N2 -(6, 7-dimethoxy-2-naphthylsulfonyl)L-arginyl-N-(2-methoxypropyl)glycine as an amorphous solid.

1. concentrationAt the end of this period, the reaction mixture was concentrated
2. customchromatographed on 200 ml of Daiaion® SK 102 ion exchange resin (200-300 mesh, H+ form, manufactured by Mitsubishi Chemical Industries Limited)
3. washwashed with ethanol-water (1:4)
4. washeluted with ethanol-water-NH4OH (10:9:1)
5. customThe main fraction was evaporated to dryness
6. washwashed with ether