2,4-Dinitrophenol → HCID 178512180, compound

Participants

Reaction Steps

In 30 ml. of DMF was dissolved in 1.0 g. of Z-β-Ala-Phe-Phe-Tyr-NH2, and using Pd-black as a catalyst, catalytic reduction was carried out for 6 hours. The catalyst was filtered off. To the filtrate was added a solution of Z-Arg(NO2)-ODNP in tetrahydrofuran (the dinitrophenyl ester prepared from 0.52 g. of Z-Arg(NO2)-OH and 0.30 g. of 2,4-dinitrophenol in 10 ml. of tetrahydrofuran by the DCC process). The mixture was stirred for 13 hours and the solvent was distilled off. To the residue was added 50 ml. of water and the resulting powder was collected by filtration and dried. Yield 1.01 g.(74.9 %); melting point: 192°-194°; α!D22 -11.1° (c=1.02 %, DMF)

Provenance & Evidence