Glycine → HCID 178512888, compound

Participants

Reaction Steps

In 6-ml. of anhydrous hydrogen fluoride was dissolved 500 mg. of Z-Phe-Arg(NO2)-Gly-Phe-Phe-NH2 and the solution was stirred at -10° C for 40 minutes. The hydrogen fluoride was distilled off and the residue was dissolved in 10 ml. of water. The solution was passed through a column (0.9 × 15 cm) of Amberlite IRA-410(acetate-form) which was washed with water. The effluent was combined with the washings and the combined solution was lyophilized. The procedure yielded 402 mg. of the above-indicated compound. Amino acid analysis: Arg, 1.01; Gly, 0.98; Phe, 2.94 (peptide content 86.2%).

Provenance & Evidence