Iodine → HCID 131721381, compound

Participants

Reaction Conditions

Reaction Steps

To a solution of tetrakis(triethylammonium)-2'-amino,2'-deoxyuridine 5'-triphosphate (35.2 mg, 0.02 mmoles, 0.02M) in sodium acetate (0.1M, 2 ml, pH 6.0) was added a solution of mercuric acetate (63.74 mg in 0.1M sodium acetate, 0.01M). The mixture was heated at 50° C. for 3 h. After cooling to room temperature, a solution of iodine in ethanol (25.38 mg I2 in 80 μl ethanol) was added. The mixture was stirred at room temperature in the dark for 1 h. The product was purified by DEAE Sephadex-A25 (Pharmacia) chromatography (0-1M triethyl ammonium bicarbonate buffer). To remove excess buffer salt the product was repeatedly concentrated from methanol to yield 6 mg (30%) tetrakis(triethylammonium)-5-iodo,2'-amino,2'-deoxyuridine 5'-triphosphate. UV: λmax 273 nm.

Provenance & Evidence