Water → Indole

Participants

Reaction Steps

Active carbon and water were added to a reacton fluid containing L-tryptophan obtained by condensing indole with L-serine in an aqueous medium in the presence of tryptophan synthase as an enzyme prepared by culturing Escherichia coli, followed by making the pH of the mixture 4 with sulfuric acid, heating at 95°~100° C. for one hour to convert the fungus body into flocs, and thereafter removing the fungus body adsorbed onto the active carbon, as it was, by filtration. The aqueous solution (2,580 g; the total mol equivalent of amino acids and cations, 1.9) obtained by the above pretreatment and containing L-tryptophan (3.8%, 0.19 M), indole (0.1%), L-serine (0.2%, 0.02 M), ammonium ion (0.8%, 0.44 M) and sodium ion (0.2%, 0.09 M) was passed through a column filled with 1 liter of Lewatit SP-112 (tradename of product manufactured by Bayer Company; exchange capacity, 1.9 mol/l) subjected to conditioning regeneration, from the upper part of the column, at a SV of 5. The flowing down fluid after completion of the passage was subjected to to detection of L-tryptophan according to a high speed liquid chromatography. It was not detected.

Provenance & Evidence