HRID622623
反应详情
EQUATION
反应方程式
REACTANTS
反应物
PRODUCTS
生成物
AUXILIARIES
试剂、催化剂与溶剂
PROCEDURE
实验过程
Benzhydrylamine-resin (BHA) (20 g, 0.5-0.7 meg/g) was coupled with Boc-Cys(Dmb)-OH (13.24g, 40.7mmol) in CH2Cl2 (250 mL) with dicyclohexyl carbodiimide (DCC) (8g,) for 4 hours. The resultant Boc-Cys(Dmb)-BHA- resin was washed with CH2Cl2 (3×300mL), DMF (2×300mL), MeOH (3×300mL), CH2Cl2 (3×300mL) and dried. An aliquot was hydrolyzed (1mL of 6M propionic HCl at 130° for 2 hours). Amino acid analysis showed a substitution of 0.52 mmol of Cys per gram of resin. The remaining amino groups were acetylated with AC2 0-pyridine - yield: 28.75g
WORKUP
后处理
- washThe resultant Boc-Cys(Dmb)-BHA- resin was washed with CH2Cl2 (3×300mL), DMF (2×300mL), MeOH (3×300mL), CH2Cl2 (3×300mL)
- customdried
- custom(1mL of 6M propionic HCl at 130° for 2 hours)