反应详情

Dowex 50WX8, 100-200 mesh (0.48 g, 6.43 μmol) resin in 2 mL water was loaded into a cartridge and let water run off. Trimethylammonium (2S,5R)-3-(((tert-butyldimethylsilyl)oxy)methyl)-2-carbamoyl-7-oxo-1,6-diazabicyclo[3.2.1]oct-3-en-6-yl sulfate sodium salt (Intermediate 166, 3 mg, 6.43 μmol) in water was passed through the resin 2 times (pH is ˜3). The solution was carried forward to the final ion exchange column, see Example 18.