Uracil → D-xylonic acid

Participants

Reaction Steps

The resulting strains H3679 and H3680 were cultured on SC-medium lacking uracil for plasmid selection, with 10 g I−1 glucose and 20 g I−1 xylose as carbon sources. The strains were cultured in 250 ml Erlenmeyer flasks in 50 ml medium with 250 rpm agitation at 30° C. Strain H3679 expressing the gene CC_1225 produced 3.3 g xylonic acid I−1 extracellularly in 75 h (FIG. 14). Strain H3680 (control strain) produced no xylonic acid when grown under the same conditions.

Provenance & Evidence