HuagongsheHuagongshe Chemistry Print Report · For research reference only
L-Serine → Arachidonic Acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | L-Serine | HCID 5951 | — |
| Reactant | L-Aspartic Acid | HCID 5960 | — |
| Reactant | L-Lysine | HCID 5962 | — |
| Reactant | L-Tyrosine | HCID 6057 | — |
| Reactant | L-Leucine | HCID 6106 | — |
| Reactant | L-Methionine | HCID 6137 | — |
| Reactant | L-Phenylalanine | HCID 6140 | — |
| Reactant | L-Valine | HCID 6287 | — |
| Reactant | L-Threonine | HCID 6288 | — |
| Reactant | L-Tryptophan | HCID 6305 | — |
| Reactant | L-Glutamic Acid | HCID 33032 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | 321-30-2 | HCID 118738 | — |
| Reactant | HCID 178418600 | HCID 178418600 | — |
| Reactant | HCID 178419078 | HCID 178419078 | — |
| Product | Arachidonic Acid | HCID 444899 | — |
Reaction Steps
ARA3-1 strain was transformed with plasmids pYE22m, pYE-MALPLAT5, and pYE-MALPLAT6. Transformed strains were selected by viability on SC-Trp, Leu, Ura agar medium (2% agar) containing, per liter, 6.7 g Yeast nitrogen base w/o amino acids (DIFCO), 20 g glucose and 1.3 g amino acid powder (a mixture of 1.25 g adenine sulfate, 0.6 g arginine, 3 g aspartic acid, 3 g glutamic acid, 0.6 g histidine, 0.9 g lysine, 0.6 g methionine, 1.5 g phenylalanine, 11.25 g serine, 0.9 g tyrosine, 4.5 g valine, and 6 g threonine).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US09315835B2 |
| Source dataset | uspto-grants-2016_04 |
| ORD ID | ord-93b919772abc4c698d0b217648e1d666 |
| Source note | uspto-grants-2016_04 |