反应详情

温度

25 °C

NaOH (71.9 mg, 1.8 mmol) was added to trans-[4-(4-{3-[(1-phenyl-3-trifluoromethyl-1H-pyrazole-4-carbonyl)amino]propionylamino}phenyl)cyclohexyl]acetic acid methyl ester (100 mg, 0.18 mmol) dissolved in THF/MeOH/water (25 mL, 3:2:1). The reaction mixture was stirred at 25° C. for 5 hours and concentrated under vacuum. The residue was acidified with 1M HCl up to pH of 2 and extracted with ethyl acetate. The extract was washed with brine and dried with anhydrous sodium sulfate and concentrated under vacuum. Acetonitrile was added thereto and cooled to obtain a filtrate, and the filtrate was filtered and separated to obtain the desired compound (95 mg, 98%) as a white solid.

1. concentrationconcentrated under vacuum
2. extractionextracted with ethyl acetate
3. washThe extract was washed with brine
4. dry with materialdried with anhydrous sodium sulfate
5. concentrationconcentrated under vacuum
6. additionAcetonitrile was added
7. temperaturecooled
8. customto obtain a filtrate
9. filtrationthe filtrate was filtered
10. customseparated