Acetonitrile → [2-(2,6-Dioxo-piperidin-3-YL)-1,3-dioxo-2,3-dihydro-1H-isoindol-5-ylmethyl]-carbamic acid hexyl ester

HRID 63404Notes

Reaction equation

HRID 63404 reaction equation
Cl.[NH2:2][CH2:3][C:4]1[CH:5]=[C:6]2[C:10](=[CH:11][CH:12]=1)[C:9](=[O:13])[N:8]([CH:14]1[CH2:19][CH2:18][C:17](=[O:20])[NH:16][C:15]1=[O:21])[C:7]2=[O:22].Cl[C:24]([O:26][CH2:27][CH2:28][CH2:29][CH2:30][CH2:31][CH3:32])=[O:25].C(N(CC)CC)C.CC#N>C1COCC1.C(OCC)(=O)C>[CH2:27]([O:26][C:24](=[O:25])[NH:2][CH2:3][C:4]1[CH:5]=[C:6]2[C:10](=[CH:11][CH:12]=1)[C:9](=[O:13])[N:8]([CH:14]1[CH2:19][CH2:18][C:17](=[O:20])[NH:16][C:15]1=[O:21])[C:7]2=[O:22])[CH2:28][CH2:29][CH2:30][CH2:31][CH3:32]

Participants

7

Reactant 4

HCID 6342AcetonitrileHCID 8471TriethylamineHCID 22466Hexyl chloroformateHCID 670197065-(Aminomethyl)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione hydrochloride

Product 1

HCID 24788975[2-(2,6-Dioxo-piperidin-3-YL)-1,3-dioxo-2,3-dihydro-1H-isoindol-5-ylmethyl]-carbamic acid hexyl esterYield 31%

Reagents, catalysts & solvents 2

HCID 8028TetrahydrofuranSolventHCID 8857Ethyl AcetateSolvent

Conditions

Temperature
40 °C

Procedure

A mixture of 5-aminomethyl-2-(2,6-dioxo-piperidin-3-yl)-isoindole-1,3-dione hydrochloride (1.0 g, 3.1 mmol), hexyl chloroformate (0.51 g, 3.1 mmol), and triethylamine (0.63 g, 6.2 mmol) in THF (35 mL) was stirred at 40° C. under nitrogen for 3 hours. The mixture was cooled and diluted with ethyl acetate (100 mL), washed with water (3×100 mL), and evaporated. The residue was chromatographed in methylene chloride-methanol gradient, eluting the product at 19:1 methylene chloride-methanol. This material was further purified by preparative HPLC, using a 50-50 acetonitrile-water isocrat, and providing 0.40 g as a white solid, in 31% yield; mp 111-113° C.; HPLC, Waters Symmetry C-18, 3.9×150 mm, 5 μm, 1 mL/min, 240 nm, 50/50 CH3CN/0.1% H3PO4, 5.08 (99.11%); 1H NMR (DMSO-d6) δ 1.11-1.57 (m, 11H), 2.02-2.07 (m, 1H), 2.46-2.63 (m, 2H), 2.84-2.96 (m, 1H), 3.97 (t, J=8.3 Hz, 2H), 4.35 (d, J=6.0 Hz, 2H), 5.15 (dd, J=12.9 Hz, J=5.4 Hz, 1H), 7.72-7.77 (m, 2H), 7.84-7.90 (m, 2H), 11.13 (s, 1H); 13C NMR (DMSO-d6) δ 13.8, 22.0, 25.0, 28.6, 30.8, 30.9, 43.5, 49.0, 64.0, 121.6, 123.5, 129.8, 131.6, 133.2, 147.9, 156.6, 167.0, 167.1, 169.8, 172.7; Anal. Calcd for C21H15N3O6: C, 60.71; H, 6.07; N, 10.11. Found: C, 60.49; H, 6.13; N, 9.91.

Workup

  1. temperatureThe mixture was cooled
  2. washwashed with water (3×100 mL)
  3. customevaporated
  4. customThe residue was chromatographed in methylene chloride-methanol gradient
  5. washeluting the product at 19:1 methylene chloride-methanol
  6. customThis material was further purified by preparative HPLC
  7. customproviding 0.40 g as a white solid

Sources & evidence

ORD record
ord-3e491369d2a84258b3932730efa88f99
Source dataset
uspto-grants-2016_09
Patent number
US09447070B2

Reaction notes

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