HuagongsheHuagongshe Chemistry Print Report · For research reference only
Water → Deoxyuridine Triphosphate
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Water | HCID 962 | — |
| Reactant | 5-(3-Amino)allyldeoxyuridine 5'-triphosphate | HCID 86599380 | — |
| Solvent | Dimethyl Sulfoxide | HCID 679 | — |
| Solvent | Sodium carbonate bicarbonate | HCID 86624777 | — |
| Product | Deoxyuridine Triphosphate | HCID 65070 | — |
Reaction Steps
Dye-PC NHS ester (3 equivalents) dissolved in DMSO or other water miscible solvent is added to a solution of 5-(3-aminoallyl)-2′-deoxyuridine-5′-triphosphate in 0.1M sodium carbonate/bicarbonate buffer (pH 8.5). Mixture is stirred at room temperature for 2-3 h and crude reaction is purified by reversed-phase HPLC.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US09322051B2 |
| Source dataset | uspto-grants-2016_04 |
| ORD ID | ord-f39235db8c08411b9ead5475f7444253 |
| Source note | uspto-grants-2016_04 |