HCID 118131231, compound → HCID 118131232, compound

Participants

Reaction Steps

Racemate 3-(1-(4-amino-3-(3-fluoro-5-hydroxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl)-4-(4-(3-(dimethylamino)propyl)phenyl)-1H-isochromen-1-one formate (example 138, 0.010 g, 0.016 mmol) was dissolved in Ethanol/Methanol 1/1 (3 ml) and submitted to chiral resolution by Chiral preparative chromatography. Conditions: Column: Chiralpak IC (25×2.0 cm), 5μ; Mobile phase: n-Hexane/(2-Propanol/Methanol 1/1+0.1% isopropylamine) 60/40% v/v; Flow rate: 16 ml/min; DAD detection: 220 nm; Loop: 1000 μl; Injection: 3.3 mg (each injection).

Provenance & Evidence