HCID 123310675, compound → HCID 91809170, compound

Participants

Reaction Steps

3-(1-(4-amino-3-(3-((tert-butyldimethylsilyl)oxy)-5-fluorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl)-4-phenyl-1H-isochromen-1-one single enantiomer (intermediate Q.3, second eluted enantiomer under the conditions described above, 0.038 g, 0.063 mmol) was dissolved in a solution of 1M HCl in EtOH (0.2 mL) and stirred at room temperature for 5 hrs. The volatiles were removed under reduced pressure and the residue was purified by flash chromatography on silica gel cartridge (DCM to DCM:MeOH=97:3) to afford title compound as a white solid (0.025 g, 0.05 mmol, 80%). This compound proved to be the first eluted enantiomer under Chiral HPLC conditions of Method A5: Rt=7.3 min, ee>99%.

Provenance & Evidence