4-Nitrobenzoyl chloride → 5'-O-p-Nitrobenzoyl-2-N-t-Butylphenoxyacetyldeoxyguanosine

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Reaction Steps

The synthesis of this product is carried out by reacting 1.1 eq of p-nitrobenzoyl chloride with 2-N-t-butylphenoxyacetyldeoxyguanosine for 16 hours in pyridine. After extraction with a dichloromethane/methanol mixture and evaporation to dryness, the product is taken up in chloroform/hexane (8/2) and purified on a silica column (elution 3% methanol). The product obtained is very soluble in organic solvents.

Provenance & Evidence