反应详情

Into a suspension of methyl 4-{[(4-{[2-(5-iodo-2-oxo-1,2dihydro-3H-indol-3-ylidene)hydrazino]carbonyl}phenyl)amino]carbonyl)benzoate (546 mg, 0.96 mmol) in THF/H2O (2/1) (20 mL) was added NaOH (1.0 N, 4.8 mL). The resulting solution was stirred at rt for 2 hr and quenched with HCl (5 N, 4 mL). A yellow solid precipitated out. Filtration, washing with water (4×5 mL) and drying under vacuo at 70° C. for 20 hrs gave the title compound as a yellow powder (451 mg, 86%) in 97.0% purity by HPLC (MaxPlot detection between 230 and 400 nm).

1. customquenched with HCl (5 N, 4 mL)
2. customA yellow solid precipitated out
3. filtrationFiltration
4. washwashing with water (4×5 mL)
5. customdrying under vacuo at 70° C. for 20 hrs