Sorbosone, L- → L-(-)-Sorbose

Participants

Reaction Steps

The reaction (200 μl) with the membrane fraction (100 μl) was carried out in 50 mM potassium phosphate buffer (pH7.0), 30° C. with shaking at 220 rpm for 15 h. The substrates tested were L-sorbosone (1% final concentration) and L-sorbose (2% final concentration). The final protein concentration used in the reaction was 1.5 mg/ml. At the end of the incubation period, 680 mg/l and 10 mg/l of L-ascorbic acid had been produced from 1% L-sorbosone and 2% L-sorbose, respectively.

Provenance & Evidence