4-Methyl-L-glutamate → gamma-methyl-L-glutamic acid gamma-hexyl-L-glutamic acid

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Reaction Steps

The synthesized copolymer (10 mg) was dissolved in deuterated trifluoroacetic acid, and the 1H NMR was measured. As a result, a peak derived from the methyl group of γ-methyl-L-glutamic acid was detected near 3.8 ppm, and a peak derived from the proton at the α-position of γ-methyl-L-glutamic acid and γ-hexyl-L-glutamic acid was detected near 4.7 ppm. When peak area near 3.8 ppm is A and that near 4.7 ppm is B, A/B=1.17. Since A/B=3.00 for poly-γ-methyl-L-glutamic acid, the decreased portion is considered to be substituted by a hexyl group. Therefore, the content percentage of the hexyl group is 61% since 1.83/3.00=0.61. Hence, the composition of the copolymer is γ-methyl-L-glutamic acid (39%)/γ-hexyl-L-glutamic acid (61%).

Provenance & Evidence