反应详情

A solution of (2R,3S,5R,6R) 2-(N-acetyl)carbamoyloxymethyl-2-methyl-6-phenoxyacetamidopenam-3-carboxylic acid p-nitrobenzyl ester (4.68 gm, 8 mmols) in dry methylene chloride (40 ml) was cooled to -60° under nitrogen. It was charged with dimethylaniline (4.06 gm, 33.5 mmol) and phosphorus pentachloride (3.68 gm, 17.7 mmol) and stirred for 30 minutes at -64° to -56°, during which time the phosphorus pentachloride dissolved. A solution of dry methanol (8.10 ml) in dry methylene chloride (8.10 ml) was then slowly added at -55° to -45°. The reaction mixture was stirred for 20 minutes at -60° to -55° and then poured into 16 ml of ice-water. The pH was adjusted to 1.7 with concentrated ammonia and the layers were separated. The methylene chloride layer was washed with 20 ml of pH 2.0 buffer which was separated and combined with the aqueous layer. Methylene chloride (20 ml) was added to the combined aqueous layers and the mixture was adjusted to pH 6.5 with concentrated ammonia. The phases were separated, the water was washed with 20 ml of methylene chloride, and the combined methylene chloride layers were dried over molecular sieves. After filtration, the solution was concentrated in vacuo to about 20 ml and then added dropwise to 400 ml of stirring heptane. The heptane was decanted from the solid which precipitated and was replaced by 100 ml of fresh heptane. The suspension was thoroughly stirred, filtered, washed, air dried, and finally dried in vacuo. There was obtained 1.66 gm of the title product as a white amorphous solid. The purity was estimated from its nmr spectrum to be about 90%, the remainder being primarily the non-acetylated material.

1. dissolutiondissolved
2. customthe layers were separated
3. washThe methylene chloride layer was washed with 20 ml of pH 2.0 buffer which
4. customwas separated
5. additionMethylene chloride (20 ml) was added to the combined aqueous layers
6. customThe phases were separated
7. washthe water was washed with 20 ml of methylene chloride
8. dry with materialthe combined methylene chloride layers were dried over molecular sieves
9. filtrationAfter filtration
10. concentrationthe solution was concentrated in vacuo to about 20 ml
11. additionadded dropwise to 400 ml of stirring heptane
12. customThe heptane was decanted from the solid which
13. customprecipitated
14. stirringThe suspension was thoroughly stirred
15. filtrationfiltered
16. washwashed
17. customair dried
18. customfinally dried in vacuo