Hydrochloric Acid → HCID 131713115, compound

Participants

Reaction Steps

A mixture of 7-[2-methylthiomethoxyimino-2-(4-formamidopyrimidin-2-yl)acetamido]-3-(1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (1.9 g) and concentrated hydrochloric acid (0.7 ml) in methanol (19 ml) was stirred for 1.5 hours at room temperature. The mixture was adjusted to pH 4 with aqueous sodium bicarbonate and evaporated under reduced pressure. The residue was dissolved in water (200 ml) and subjected to column chromatography on a non ionic adsorption resin "HP-20" (300 ml). After the column was washed with water, the elution was carried out with 25% aqueous methanol. The eluate was evaporated to remove methanol under reduced pressure and lyophilized to give 7-[2-methylthiomethoxyimino-2-(4-aminopyrimidin-2-yl)acetamido]-3-(1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (1.13 g).

Provenance & Evidence