Hydrochloric Acid → HCID 131713127, compound

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Reaction Steps

A mixture of 7-[2-ethoxyimino-2-(4-aminopyrimidin-2-yl)acetamido]-3-(3-formamido-1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (430 mg) and concentrated hydrochloric acid (0.16 ml) in methanol (4.3 ml) was stirred for 2 hours at room temperature. The mixture was adjusted to pH 4 with aqueous sodium bicarbonate and evaporated under reduced pressure. The residue was dissolved in water (50 ml) and subjected to column chromatography on a non ionic adsorption resin "HP-20" (70 ml). After the column was washed with water, the elution was carried out with 25% aqueous methanol. The eluate was evaporated to remove methanol under reduced pressure and lyophilized to give 7-[2-ethoxyimino-2-(4-aminopyrimidin-2-yl)acetamido]-3-(3-amino-1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (0.22 g), mp 140° to 145° C. (dec.).

Provenance & Evidence