HRID653385

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=C(O)C(CCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2O)N1C(=O)CCC1=O

HCID129676281

未命名化合物

PRODUCTS

生成物

结构：O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21

HCID171548

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID679

Dimethyl sulfoxide

C2H6OS

PROCEDURE

实验过程

Dissolve 1 mg of N-hydroxysuccinimidobiotin (bio-NHS) in 1 ml dimethylsulfoxide. Add 7 ul of bio-NHS solution to protein (a 3 fold molar excess). Let sit 1 hr at room temperature. Dialyze vs PBS.

WORKUP

后处理

customDialyze vs PBS