反应详情

After completion of the cultivation, the cells were collected by centrifugation, washed and a sterilized water was added thereto to obtain 250 ml of a cell suspension. Into 750 ml of an aqueous solution containing 66.7 mM 1,2,4-triazole-3-carboxamide, 66.7 mM inosine and 100 mM monopotassium dihydrogen phosphate was added 250 ml of the above cell suspension, and the reaction was carried out at 60° C. for 24 hours (ribavirin yield: 74.88%).