Dichloromethane → Tobramycin

Beteiligte Stoffe

Reaktionsschritte

To a solution of 75 ml of dichloromethane was added 10 grams of soy lecithin (with tracer amounts of 3H-phosphatidlylcholine) and 200 mg of tobramycin sulfate in 5 ml of water. The solution was mixed by shaking and 4 ml were applied onto each side of 1"×1" foam bandage material and allowed to air dry. The bandage materials were placed onto a grid support as in Example 1 and wetted with 5 ml of water. The eluents for the first nine wettings were discarded. After ten repeated wettings the eluent was collected and passed over a Sephadex G-150 column. The material which was excluded by the column and included by the column bed was assayed for tobramycin using standard antimicrobial assays with E. Coli strain Y-1089. The material which was excluded was subjected to turbidity measurements and laser light scattering analysis. The sizes of the SDMCs were consistent with those previously observed between 0.15 μm and 0.3 μm and when assayed for tobramycin demonstrated that the antibiotic was indeed entrapped within the SDMCs which eluted from the foam bandage material. The included volume contained a small amount (less than 10% of the total tobramycin) which had either not been entrapped in SDMCs or had leaked out of SDMCs during transit from foam dressing or over the Sephadex column.

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