反应详情

To a solution of crude (2S,4R)-tert-butyl 4-(((9H-fluoren-9-yl)methoxy)carbonylamino)-2-((4-ethylphenylcarbamoyloxy)methyl)pyrrolidine-1-carboxylate in MeOH was added HCl (4 N in dioxane). The reaction was stirred at rt for 1 h and concentrated. The residue was dissolved in THF (10 mL). To this solution were added DIEA (469 μL, 2.7 mmol) and 2-chlorobenzylisocyanate. The mixture was stirred at rt for 1 h and concentrated. The residue was dissolved in 10 ml of 20% piperidine in DCM. The reaction mixture was stirred at rt for 15 min, concentrated and purified on RP-HPLC using a mixture of acetonitrile and H2O to give ((2S,4R)-4-amino-1-(2-chlorobenzylcarbamoyl)pyrrolidin-2-yl)methyl 4-ethylphenylcarbamate (210 mg, 53% for 4 steps). LRMS (M+H+) m/z 431.1.

1. concentrationconcentrated
2. dissolutionThe residue was dissolved in THF (10 mL)
3. stirringThe mixture was stirred at rt for 1 h
4. concentrationconcentrated
5. dissolutionThe residue was dissolved in 10 ml of 20% piperidine in DCM
6. stirringThe reaction mixture was stirred at rt for 15 min
7. concentrationconcentrated
8. custompurified on RP-HPLC
9. additiona mixture of acetonitrile and H2O