Hydrochloric Acid → HCID 57819937, compound

Participants

Reaction Conditions

Reaction Steps

174(d) 1,1-Dimethylethyl (2S)-4-{[5′-({[4-({[1,6-diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]methyl}amino)-3,3-dimethyl-4-oxobutanoyl]amino}methyl)-2′-fluoro-3-biphenylyl]methyl}-2-methyl-1-piperazinecarboxylate was dissolved in 2 mL of dioxane and 0.5 mL of MeOH. HCl (concentrated, 101 mg, 2.78 mmol) was added to the resultant solution and heated at 60° C. for 1 h. The solution was applied to an amine column, rinsed with 4 mL of MeOH to afford 7 mg of the title compound (3.5%). LC-MS m/z 727 (M+H)+, 1.23 min (ret time). The structure of the title compound was confirmed by gCOSY45, gHMQC, gHMBC and 13C gaspe spectra.

Provenance & Evidence