HCID 178477074, compound → HCID 178512856, compound

HRID 65867Notes

Reaction equation

HRID 65867 reaction equation
FC(F)(F)C(O)=O.C([S:11][CH2:12][CH:13]([CH2:28][CH2:29][CH2:30][NH2:31])[C:14]([NH:16][C@H:17]([C:25]([OH:27])=[O:26])[CH2:18][CH2:19][CH2:20][NH:21][C:22](=[NH:24])[NH2:23])=[O:15])(=O)C>O.N>[NH2:31][CH2:30][CH2:29][CH2:28][CH:13]([CH2:12][SH:11])[C:14]([NH:16][C@H:17]([C:25]([OH:27])=[O:26])[CH2:18][CH2:19][CH2:20][NH:21][C:22](=[NH:23])[NH2:24])=[O:15]

Participants

4

Reactant 1

HCID 178477074HCID 178477074, compound

Product 1

HCID 178512856HCID 178512856, compound

Reagents, catalysts & solvents 2

HCID 222AmmoniaSolventHCID 962WaterSolvent

Procedure

Nα -(2-acetylthiomethyl-5-aminopentanoyl)-L-arginine trifluoroacetate (2 g) is dissolved in a mixture of water (25 ml) and concentrated ammonia (25 ml) and this solution is stored at room temperature for 20 minutes. The solution is concentrated in vacuo to ca. 5 ml and applied to a column of Dowex 50 ion exchange resin (50 ml) in the hydrogen cycle. After washing with water Nα -(5-amino-2-mercaptomethylpentanoyl)-L-arginine is eluted with a pyridine-acetate buffer at pH 6.5.

Workup

  1. concentrationThe solution is concentrated in vacuo to ca. 5 ml
  2. washAfter washing with water Nα -(5-amino-2-mercaptomethylpentanoyl)-L-arginine
  3. washis eluted with a pyridine-acetate buffer at pH 6.5

Sources & evidence

ORD record
ord-a9ee2764dbfb4dd0b67efb1c6204d582
Source dataset
uspto-grants-1978_12
Patent number
US04129571

Reaction notes

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