反应详情

6 ml of an aqueous 1N sodium hydroxide solution (6 millimols of NaOH) which had been cooled to about 0° C. was added dropwise over a period of 5 minutes to a suspension of 2.42 gm (6 millimols) of ampicillin trihydrate in 24 ml of water at about 0° C., while cooling and vigorously stirring. The resulting clear solution was immediately sterile-filtered, that is, filtered to remove suspended particles, and the filtrate was without delay frozen in bulk by the shell-freezing process. Thereafter, the frozen filtrate was lyophilized, yielding 2.2 gm of white sodium ampicillin having a residual moisture content of 1.35% by weight and a microbiological activity of 94% (U.S.P. standard).

1. temperaturewhile cooling
2. filtrationThe resulting clear solution was immediately sterile-filtered
3. filtrationfiltered
4. customto remove
5. temperaturefrozen in bulk by the shell-freezing process