Hydrochloric Acid → HCID 44193055, compound

Participants

Reaction Steps

To a solution of N-{[1,6-diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]methyl}-N′-{[6-methyl-3′-(1-piperazinylmethyl)-3-biphenylyl]methyl}-2,6-pyridinedicarboxamide (565 mg, 0.774 mmol) in ethanol (1 mL) was added 1N HCl (0.735 mL, 0.735 mmol). The mixture was stirred at RT for 2 h. It was concentrated under vacuum. The residue was re-dissolved in ethanol and stripped down (this was repeated for three times). Then a 1:1 mixture of DCM:hexane (5 mL) was added and concentrated (this was repeated twice). Finally, the white solid that formed was dried under high vacuum for two days to give N-{[1,6-diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]methyl}-N′-{[6-methyl-3′-(1-piperazinylmethyl)-3-biphenylyl]methyl}-2,6-pyridinedicarboxamide hydrochloride as a solid (530 mg, 89%). LC-MS m/z 730 (M+H)+, 0.79 min (ret time).

Provenance & Evidence