反应详情

To a mixed solution of (6S,9aS)-N-benzyl-6-((2-fluoro-4-hydroxyphenyl)methyl)-8-((6-fluoropyridin-2-yl)methyl)-4,7-dioxo-2-(prop-2-en-1-yl)-octahydro-1H-pyrazino[2,1-c][1,2,4]triazine-1-carboxamide (397 mg, 0.689 mmol) described in Production Example 1-1-6 and NMP (10 mL) was added a mixture (1.16 g) of 1-(azetidin-3-yl)-4-ethylpiperazine and benzylbenzene described in Production Example 1-3-2 at room temperature. The resultant mixture was irradiated with a microwave at 140° C. for 12 hours. The reaction mixture was cooled to room temperature, water was then added thereto, then the resultant solution was extracted with ethyl acetate, and an organic layer was washed with saturated brine. The organic layer was dried over anhydrous magnesium sulfate and then filtrated. The solvent was evaporated under a reduced pressure, and the resultant residue was purified by silica gel column chromatography (methanol) and then further purified by NH silica gel column chromatography (ethyl acetate:methanol=20:1) to give the title compound (402 mg, yield: 80%).

1. customdescribed in Production Example 1-3-2 at room temperature
2. extractionthe resultant solution was extracted with ethyl acetate
3. washan organic layer was washed with saturated brine
4. dry with materialThe organic layer was dried over anhydrous magnesium sulfate
5. filtrationfiltrated
6. customThe solvent was evaporated under a reduced pressure
7. customthe resultant residue was purified by silica gel column chromatography (methanol)
8. customfurther purified by NH silica gel column chromatography (ethyl acetate:methanol=20:1)