関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Dimethylformamide | HCID 6228 | — |
| 反応物 | 2-Sulfamoylbenzoic acid | HCID 69436 | — |
| 反応物 | N-Hydroxysuccinimide | HCID 80170 | — |
| 反応物 | Diisopropylethylamine | HCID 81531 | — |
| 反応物 | 1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride | HCID 2723939 | — |
| 生成物 | Glycyl-L-leucine | HCID 92843 | — |
反応手順
Carboxy benzene sulfonamide (20 μmol in 20 μL dry DMF (J. T. Baker), N-hydroxysuccinimide (22 μmol in 22 μL dry DMF), and EDC-HCl (14 μmol in 140 μL dry DMF) were mixed together and stirred at room temperature for 4 hours. The dipeptide H-leucine-glycine-OH (2 μmol in 20 μL dry DMF) and DIPEA (1.2 μL, 8 μmols) were added and the resulting mixture was stirred overnight at room temperature. ESI-MS calculated for [M−H+]−: 370.41 Found: 369.97. 1H NMR (DMSO-d6, 500 MHz) δ 8.72 (d, 1H, J=8.5 Hz), 8.30 (t, 1H, J=5.5 Hz), 8.09 (d, 2H, J=8 Hz), 7.94 (d, 2H, J=8 Hz), 7.52 (s, 2H), 4.61 (m, 1H), 3.77 (m, 2H), 1.72 (m, 2H), 1.62 (m, 1H), 0.95 (d, 3H, J=6.5 Hz), 0.92 (d, 3H, J=6.5 Hz). 13C NMR (DMSO-d6, 125 MHz) δ 173.0, 171.8, 166.0, 147.0, 137.7, 128.9, 126.2, 52.4, 41.5, 41.0, 25.1, 23.8, 22.0.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US09175340B2 |
|---|
| 出典データセット | uspto-grants-2015_11 |
|---|
| ORD ID | ord-ead52aa9885e4901b4010f8d687f3dff |
|---|
| 出典メモ | uspto-grants-2015_11 |
|---|
