反应详情

温度

2 °C

To (2S,5R)-methyl 6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxylate (809.0 mg, 2.79 mmol) were added tetrahydrofuran (8 mL) and water (3.6 mL), then 0.5M lithium hydroxide aqueous solution (6.41 mL) was added dropwise at 4.9° C. or less over 10 minutes. The reaction solution was stirred at 2° C. for 2 hours, followed by adding water (30 mL) and washing with ethyl acetate (25 mL). To the separated aqueous layer was added ethyl acetate (15 mL), the aqueous layer was adjusted to pH 4.0 with 1M hydrochloric acid aqueous solution, and extracted with ethyl acetate twice (ethyl acetate: total volume of 65 mL). The separated aqueous layer was adjusted to pH3.4 with 1M hydrochloric acid aqueous solution, and extracted with ethyl acetate once, subsequently the aqueous layer was adjusted to pH 2.4 and extracted with ethyl acetate twice. The resulting extraction liquid from a total of five extractions with ethyl acetate (175 mL) was washed with saturated brine (40 mL), dried over anhydrous sodium sulfate, filtrated, and then concentrated under reduced pressure. 759.1 mg of the resulting residue was diluted with ethyl acetate (5 mL), and n-hexane (3 mL) was added, and a seed crystal was inoculated for crystallization. Further, ethyl acetate/n-hexane (5/3) solution (8 mL) was added and stirred, subsequently n-hexane (20 mL) was added and stirred at 4° C. for 14 hours. The crystals were filtered, followed by washing with n-hexane (55 mL), subsequently drying under vacuum to afford 633.6 mg of the title compound as a colorless crystalline powder (yield 82%). The instrumental data of this were consistent with those of the compound of Example 9.

1. washwashing with ethyl acetate (25 mL)
2. additionTo the separated aqueous layer was added ethyl acetate (15 mL)
3. extractionextracted with ethyl acetate twice (ethyl acetate: total volume of 65 mL)
4. extractionextracted with ethyl acetate once
5. extractionextracted with ethyl acetate twice
6. extractionThe resulting extraction liquid
7. extractionfrom a total of five extractions with ethyl acetate (175 mL)
8. washwas washed with saturated brine (40 mL)
9. dry with materialdried over anhydrous sodium sulfate
10. filtrationfiltrated
11. concentrationconcentrated under reduced pressure
12. addition759.1 mg of the resulting residue was diluted with ethyl acetate (5 mL), and n-hexane (3 mL)
13. additionwas added
14. customa seed crystal was inoculated for crystallization
15. additionFurther, ethyl acetate/n-hexane (5/3) solution (8 mL) was added
16. stirringstirred
17. additionsubsequently n-hexane (20 mL) was added
18. stirringstirred at 4° C. for 14 hours
19. filtrationThe crystals were filtered
20. washby washing with n-hexane (55 mL)
21. customsubsequently drying under vacuum