反应详情

To tert-butyl 4-(2,2-difluoroethyl)-2-((2,3-dihydroxypropyl)carbamoyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (422 mg, 0.96 mmol) in THF (10 mL) was added NaIO4 (522 mg, 2.44 mmol) followed by H2O (4 mL) the reaction mixture was stirred at room temperature for 2 hours. The reaction mixture was filtered and partitioned between sat. aq. sodium bicarbonate/ethyl acetate. The aqueous layer was extracted further with ethyl acetate (3×50 mL). The organics were combined, washed with sat. aq. sodium bicarbonate (50 mL), dried over MgSO4, and concentrated in vacuo to yield tert-butyl 4-(2,2-difluoroethyl)-2-((2-oxoethyl)carbamoyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (377 mg, 96%) as a white solid, which was taken onto the next step without purification. ESI-MS m/z calc. 405.2. found 406.5 (M+1)+; Retention time: 1.32 minutes (3 min run).

1. filtrationThe reaction mixture was filtered
2. custompartitioned between sat. aq. sodium bicarbonate/ethyl acetate
3. extractionThe aqueous layer was extracted further with ethyl acetate (3×50 mL)
4. washwashed with sat. aq. sodium bicarbonate (50 mL)
5. dry with materialdried over MgSO4
6. concentrationconcentrated in vacuo