Ammonia → 4-Amino-1-(2-c-methyl-beta-d-ribofuranosyl)imidazo[4,5-d]pyridazine

Participants

Reaction Steps

The compound from Step B (590 mg, 0.96 mmol) was added to a solution of ammonia in methanol and stirred in a steel bomb at 150° C. for 6 hours. The reaction mixture was evaporated to dryness to remove methanol. The crude product was purified on silica gel reverse-phase (C18) using water as eluant to give the title compound (35 mg) as a white powder.

Provenance & Evidence