反应详情

The above diarachidonylmethyl formate (III, 0.43 g, 0.71 mmol) and KOH (100 mg) were stirred in 95% EtOH (20 mL) at room temperature under nitrogen overnight. Upon completion of the reaction, most of the solvent was evaporated. The resulting mixture was treated with 20 mL of 2M HCl solution. The aqueous phase was extracted with ether (2×30 mL). The combined ether extract was washed with water (2×25 mL), brine (25 mL), and dried over anhydrous Na2SO4. Evaporation of the solvent gave 0.44 g of IV as pale oil. The crude product was purified by column chromatography on silica gel (230-400 mesh, 40 mL) eluted with 0-5% ethyl acetate gradient in hexanes. This gave 0.41 g of diarachidonyl methanol (IV) as colourless oil.

1. customUpon completion of the reaction
2. custommost of the solvent was evaporated
3. additionThe resulting mixture was treated with 20 mL of 2M HCl solution
4. extractionThe aqueous phase was extracted with ether (2×30 mL)
5. extractionThe combined ether extract
6. washwas washed with water (2×25 mL), brine (25 mL)
7. dry with materialdried over anhydrous Na2SO4
8. customEvaporation of the solvent