huagongshe.com
HRID716173

反应详情

EQUATION

反应方程式

HRID 716173 的结构方程式

REACTANTS

反应物

1
结构：CC(=O)N[C@H]1[C@@H](OP(=O)(O)OP(=O)(O)OC[C@H]2O[C@@H](n3ccc(=O)[nH]c3=O)[C@H](O)[C@@H]2O)O[C@H](CO)[C@H](O)[C@@H]1O
HCID23700

UDP-N-acetylgalactosamine

C17H27N3O17P2

PRODUCTS

生成物

1
结构：CC(=O)N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O
HCID35717

N-[(3R,4R,5R,6R)-2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide

C8H15NO6

AUXILIARIES

试剂、催化剂与溶剂

1
结构：OCCN(CCO)C(CO)(CO)CO
溶剂HCID81462

2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol

C8H19NO5

PROCEDURE

实验过程

10 mcg of FGF-21 polypeptide (1 mg/ml) were incubated in 20 mM BisTris pH 6.7, 50 mM NaCl, 10 mM MnCl2 containing 10 molar equivalents (0.4 mM) of UDP-GalNAc and MBP-hGalNAcT2 (40 mU/mg) for 6 hours at 30° C.

WORKUP

后处理

  1. customfor 6 hours
  2. customat 30° C.