Reaction Steps
A 50 mL 3 necked round bottom flask with stir bar, nitrogen line, septum and thermometer was charged with rac-(2′R,4aS,12bS)-12b-ethyl-9-(4-fluorophenyl)-2,4,4a,5,6,7,9,12b-octahydro-1H-spiro[benzo[6,7]cyclohepta[1,2-f]indazole-3,2′-oxirane](14, R1=4-Fluorophenyl, R2=Ethyl) (0.225 g, 0.576 mmol), THF (10 mL) and copper(I) iodide (0.016 g, 0.086 mmol). The mixture was stirred for about 10 min then cooled to about −5° C. Ethylmagnesium bromide (3 M in ether, 0.576 mL, 1.73 mmol) was added keeping the internal temperature below about 0° C. After about 15 min sat. aqueous NH4Cl (3 mL), water (25 mL) and EtOAc (25 mL) were added to the mixture. The layers were separated then the organic solution was dried over MgSO4, filtered and concentrated under reduced pressure. The residue was purified on silica gel (12 g) using a gradient of 0-20% EtOAc in DCM. Product fractions were combined and concentrated under reduced pressure to yield (3S,4aR,12bR)-12b-Ethyl-9-(4-fluoro-phenyl)-3-propyl-1,2,3,4,4a,5,6,7,9,12b-decahydro-9,10-diaza-benzo[3,4]cyclohepta[1,2-f]inden-3-ol; compound with (3R,4aS,12bS)-12b-ethyl-9-(4-fluoro-phenyl)-3-propyl-1,2,3,4,4a,5,6,7,9,12b-decahydro-9,10-diaza-benzo[3,4]cyclohepta[1,2-f]inden-3-ol (16, R1=4-Fluorophenyl, R2=Ethyl, R3=Propyl) (Example 4) (0.187 g, 77%); LC/MS, method 2, Rt=3.84 min, MS m/z 421 (M+H)+. 1H NMR (400 MHz, DMSO) δ 8.27 (d, J=0.6 Hz, 1H), 7.92 (s, 1H), 7.83-7.77 (m, 2H), 7.54 (s, 1H), 7.45-7.35 (m, 2H), 3.83 (s, 1H), 3.22-3.15 (m, 1H), 2.95-2.89 (m, 1H), 2.22-2.14 m, 1H), 2.12-1.93 (m, 3H), 1.88-1.71 (m, 2H), 1.68-1.59 (m, 1H), 1.56-1.48 (m, 2H), 1.44-1.24 (m, 8H), 0.88 (t, J=6.6 Hz, 3H), 0.35 (t, J=7.3 Hz, 3H)
