反应详情

温度

22 °C

J1) To a stirred solution of methyl (2R)-4-[(benzyloxy)imino]-2-[(tert-butoxycarbonyl)-amino]butanoate (1.7 g, 5.0 mmoles) and dry methanol (50 mL) was added sodium cyanoborohydride (0.31 g, 5.0 mmoles) followed dropwise addition of a 1M solution of 4-toluenesulfonic acid (N.B. crystal water present in commercial 4-toluenesulfonic acid has to be removed by azeotropic evaporation with benzene or toluene prior to use) in dry methanol at such a rate so as to keep pH of the mixture at 3–4 (moist indicator paper). After stirring at 22° C. for 2.5 hours TLC and LC indicated a complete reaction. The methanol was stripped off by rotary evaporation leaving a white solid which was taken up in ethyl acetate (100 mL), washed with saturated sodium bicarbonate solution and brine and dried over anhydrous sodium sulfate. Filtration and concentration by rotary evaporation gave 1.7 g of a crude which was chromato-graphed on silica with ethyl acetate/n-heptane (1:5 through 1:3) as eluents. To rid the product from traces of O-benzylhydroxylamine it was chromatographed once more on silica using dichloromethane and dichloromethane/methanol (98:2) as eluents to afford 0.83 g (49% yield) of the subtitle compound as a colourless oil. MS (APCI) m/z 339 (M+1). 1H NMR (CDCl3) δ 7.40–7.27 (m, 5H), 5.47 (br d, J=8 Hz, 1H), 4.74 (s, 2 H), 4.41–4.31 (m, 1H), 3.72 (s, 3H), 3.10–2.93 (m, 2H), 2.20–2.05 (m, 1H), 1.88–1.78 (m, 1H) and 1.43 (s, 9H) ppm.

1. customto be removed by azeotropic evaporation with benzene or toluene
2. customa complete reaction
3. customThe methanol was stripped off by rotary evaporation
4. customleaving a white solid which
5. washwashed with saturated sodium bicarbonate solution and brine
6. dry with materialdried over anhydrous sodium sulfate
7. filtrationFiltration and concentration
8. customby rotary evaporation
9. customgave 1.7 g of a crude which
10. customwas chromatographed once more on silica