反应详情

温度

25 °C

A solution of 8-(4-amino-benzyl)-3-cyclopropylmethyl-1-(2-fluoro-benzyl)-3,7-dihydro-purine-2,6-dione (50 mg, 0.12 mmol) in N,N-dimethylformamide at 25° C. was treated with a solution of 1-methyl-1H-pyrazole-4-carboxylic acid (15 mg, 0.12 mmol), O-benzotriazol-1-yl-N,N.N′N′-tetramethyluronium hexafluorophosphate (49.7 mg, 0.13 mmol), and N,N-diisopropylethylamine (62 μL, 0.36 mmol). The resulting solution was stirred at 25° C. for 18 h. At this time, the reaction was concentrated in vacuo. The residue was dissolved in chloroform and then was washed with a 1N aqueous hydrochloric acid solution (1×10 mL) and a saturated aqueous sodium chloride solution (1×10 mL). The organics were dried over magnesium sulfate, filtered, and concentrated in vacuo. Flash chromatography (Merck Silica gel 60, 230–400 760mesh, 5:95 methanol/dichloromethane) afforded 1-methyl-1H-pyrazole-4-carboxylic acid {4-[3-cyclopropylmethyl-1-(2-fluoro-benzyl)-2,6-dioxo-2,3,6,7-tetrahydro-1H-purin-8-ylmethyl]-phenyl}-amide (13.5 mg, 21.5%) as a tan solid: EI-HRMS m/e calcd for C28H26N7O3 (M+) 527.2081, found 527.2083.

1. concentrationAt this time, the reaction was concentrated in vacuo
2. dissolutionThe residue was dissolved in chloroform
3. washwas washed with a 1N aqueous hydrochloric acid solution (1×10 mL)
4. dry with materialThe organics were dried over magnesium sulfate
5. filtrationfiltered
6. concentrationconcentrated in vacuo