反应详情

温度

20 °C

111 mg of N-tert-butoxycarbonyl-2(R,S)-methyl-4(S)-hydroxy-5(S)-amino-7(S)-isopropyl-8-(p-tert-butyl-phenyl)-octanoic acid (N-butyl)amide are dissolved in 2 ml of 4N hydrochloric acid in dioxane at 0° C. and then stirred for 60 minutes at 20° C. The reaction mixture is concentrated by evaporation under reduced pressure and the residue is purified by means of FC (50 g of silica gel, dichloromethane/methanol=9:1). The title compound is obtained in the form of a diastereoisomeric mixture: Rf (dichloromethane/methanol=9:1)=0.20; Rt (I)=36.6 and 37.5 minutes; FAB-MS (M+H)+ =419.

1. concentrationThe reaction mixture is concentrated by evaporation under reduced pressure
2. customthe residue is purified by means of FC (50 g of silica gel, dichloromethane/methanol=9:1)