Methanol → 5-TFA-ap-ddU

Beteiligte Stoffe

Reaktionsschritte

Iodouridine 47 was coupled for 3 h to N-propargyltrifluoroacetamide following the general method given in Example 1C. Chomatography with a 0-5% methanol in dichloromethane gradient afforded material which was homogeneous by TLC, but which was difficult to dry. After co-evaporating the chomatographed product several times with chloroform and vacuum-drying, 536.5 mg of alkynylamino nucleoside 48 was obtained as a white foam. This material was homogeneous by TLC and was pure by NMR except for a small amount (39 mole%; corrected yield 66%) of chloroform.

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