Hydrazine hydrate (1:1) → HCID 53942049, compound

Participants

Reaction Steps

A solution of approximately 0.5 mmole 3-PHT-P AZT (Example 26) in methanol was treated with 50 ul hydrazine hydrate and incubated overnight at room temperature. The resulting suspension was filtered through a 0.45 um PTFE membrane and the filtrate chromatographed by preparative TLC on silica gel-F 2000 um using the solvent system CHCl3 /MeOH/15M NH4OH (85+15+1). The major band (Rf =0.27; UV positive; ninhydrin positive)was well separated from 3-PHT-P-AZT (Rf =0.78; UV positive; ninhydrin negative) and from hydrazine (Rf =0.0; UV negative; ninhydrin postive). The product was eluted with MeOH. It was found to be immunologically active in an AZT assay by FPIA, was homogeneous on analytical TLC and its UV spectrum resembled that of a 3-alkylated AZT derivative.

Provenance & Evidence