Sodium ethoxide → HCID 137194588, compound

Participants

Reaction Conditions

Reaction Steps

A solution of the crude 2-{isopropyl-[2-(7-methanesulfonylamino-1,1-dioxo-1,4-dihydro-1λ6-benzo[1,2,4]thiadiazin-3-yl)-acetyl]-amino}-cyclopentanecarboxylic acid ethyl ester in ethanol (20 mL) was treated with a 21% w/w solution of sodium ethoxide in ethanol (0.648 g) and stirred for 16 h at 60° C. Upon cooling, the reaction mixture was then quenched with 1.0 M aqueous hydrochloric acid solution (20 mL) and extracted with ethyl acetate (2×50 mL). The combined organic layers were washed with saturated aqueous sodium bicarbonate solution, dried over sodium sulfate, filtered, and dried in vacuo. The residue was purified by prep-HPLC [Column ODS-A 5μ 100 Å, 150×21.2 mm, 5 micron, 50%-100% in 13.5 min @ 22 mL/min flow rate, 0.05% trifluoroacetic acid in acetonitrile/0.05% trifluoroacetic acid in water] to afford the desired product, cis-N-[3-(4-hydroxy-1-isopropyl-2-oxo-2,4a,5,6,7,7a-hexahydro-1H-[1]pyrindin-3-yl)-1,1-dioxo-1,4-dihydro-1λ6-benzo[1,2,4]thiadiazin-7-yl]-methanesulfonamide (77.6 mg, 0.166 mmol, 33.2% over two steps), as a white solid. 1H NMR (400 MHz, DMSO-d6) δ: 1.10-1.32 (6H, m), 1.46-2.13 (7H, m), 2.28 (1H, bs), 3.06 (3H, s), 3.95 (1H, bs), 4.54-5.10 (1H, m), 7.49-7.63 (3H, m), 10.17 (1H, s). LC-MS (ESI) calcd for C19H24N4O6S2 468.11, found 469.3 [M+H+].

Provenance & Evidence