反应详情

A 20 ml 1,4-dioxane solution of 2.01 g of 4-[(3R,4S)-3-({(tert-butoxycarbonyl)[(1R)-1-(1-naphthyl)ethyl]amino}methyl)-4-phenylpyrrolidin-1-yl]-3,5-difluorobenzoic acid was mixed with 2.0 ml of 4 M hydrogen chloride/1,4-dioxane and stirred overnight at room temperature. The reaction solution was concentrated under a reduced pressure, and the thus obtained residue was purified by a silica gel column chromatography (chloroform-methanol). The purified product was dissolved in 1,4-dioxane-diethyl ether, 4 M hydrogen chloride/1,4-dioxane was added thereto, and the thus precipitated crystals were collected by filtration and then recrystallized from 1,4-dioxane-water, thereby obtaining 484 mg of 3,5-difluoro-4-[(3S,4S)-3-({[(1R)-1-(1-naphthyl)ethyl]amino}methyl)-4-phenylpyrrolidin-1-yl]benzoic acid hydrochloride.

1. concentrationThe reaction solution was concentrated under a reduced pressure
2. customthe thus obtained residue was purified by a silica gel column chromatography (chloroform-methanol)
3. dissolutionThe purified product was dissolved in 1,4-dioxane-diethyl ether
4. addition4 M hydrogen chloride/1,4-dioxane was added
5. filtrationthe thus precipitated crystals were collected by filtration
6. customrecrystallized from 1,4-dioxane-water