huagongshe.com
HRID7341

反应详情

EQUATION

反应方程式

HRID 7341 的结构方程式

REACTANTS

反应物

2
结构：O=C([O-])O
HCID769

Bicarbonate Ion

CHO3-

结构：O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CC(S(=O)(=O)[O-])C1=O.[Na+]
HCID71571497

未命名化合物

PRODUCTS

生成物

1
结构：O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
HCID171548

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O
溶剂HCID962

Water

H2O

PROCEDURE

实验过程

About 2.0 mg of DBP is buffer exchanged with 20 mM bicarbonate at pH 9.6 three times to give a final volume of 1.0 mL. Two aliquots of sulfo-NHS-LC-biotin (Pierce) are dissolved in water to give a 1 mg/mL solution just prior to addition. The first aliquot (10 uL) is added and the reaction is maintained at room temperature for 5 minutes. The second aliquot (10 uL) is added and the reaction proceeds for an additional 4 minutes. The unreacted biotin is removed by size exclusion chromatography using SEPHADEX G-25 and 100 mM PBS, pH 7.4 as the mobile phase.

WORKUP

后处理

  1. customto give a final volume of 1.0 mL
  2. customto give a 1 mg/mL solution just
  3. additionto addition
  4. additionThe first aliquot (10 uL) is added
  5. additionThe second aliquot (10 uL) is added
  6. customThe unreacted biotin is removed by size exclusion chromatography