Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | beta-Maltose | HCID 6255 | — |
| Reactant | Panose | HCID 94448 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Product | beta-Maltose | HCID 6255 | — |
| Product | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Product | Alpha-maltotetraose | HCID 446495 | — |
Reaction Steps
As evident from the results in Table 23, it was revealed that, after the enzymatic action, glucose and α-isomaltosylglucose alias 62-O-α-glucosylmaltose or panose were mainly formed maltose as a substrate; and maltose and α-isomaltosylglucose alias 63-O-α-glucosylmaltotriose were mainly formed along with small amounts of glucose, maltotetraose, α-isomaltosylglucose alias 62-O-α-glucosylmaltose or panose, and a product X. Also, it was revealed that maltotriose and the product X were mainly formed from maltotetraose as a substrate along with small amounts of maltose, maltopentaose, α-isomaltosylglucose alias 63-O-α-glucosylmaltotriose; and a product Y; and that maltotetraose and the product Y were mainly formed from maltopentaose as a substrate along with small amounts of maltotriose, maltohexaose, and the products X and Z. The product X as a main product from maltotetraose as a substrate and the product Y as a main product from maltopentaose as a substrate were respectively isolated and purified as follows: The products X and Y were respectively purified on HPLC using “YMC PACK ODS-A R355-15S-15 12A”, a separatory HPLC column commercialized by YMC Co., Ltd., Tokyo, Japan, to isolate the product X having a purity of at least 99.9% from the reaction product from maltotetraose in a yield of about 8.3%, d.s.b., and the product Y having a purity of at least 99.9% from the reaction product from maltopentaose in a yield of about 11.5%, d.s.b.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
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| Patent number | US07592160B2 |
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| Source dataset | uspto-grants-2009_09 |
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| ORD ID | ord-228da160b19b4aee811d334461fbf76e |
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| Source note | uspto-grants-2009_09 |
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