2,3-Dihydro-2,6-pyridinedicarboxylic acid → 2,3-Dihydro-2,6-pyridinedicarboxylic acid

HRID 742184Notes

Reaction equation

HRID 742184 reaction equation
[CH2:1]1[CH:6]([C:7]([OH:9])=[O:8])[N:5]=[C:4]([C:10]([OH:12])=[O:11])[CH:3]=[CH:2]1.[CH:13]1[N:14]=[C:15]([NH2:60])[C:16]2[N:21]=[CH:20][N:19]([C@@H:22]3[O:26][C@H:25]([CH2:27][O:28][P:29]([O:32][P:33]([O:36][CH2:37][C@H:38]4[O:42][C@@H:41]([N:43]5[CH:48]=[C:47]([C:49]([NH2:51])=[O:50])[CH2:46][CH:45]=[CH:44]5)[C@H:40]([OH:52])[C@@H:39]4[OH:53])([OH:35])=[O:34])([OH:31])=[O:30])[C@@H:24]([OH:54])[C@H:23]3[O:55][P:56]([OH:59])([OH:58])=[O:57])[C:17]=2[N:18]=1>>[CH:13]1[N:14]=[C:15]([NH2:60])[C:16]2[N:21]=[CH:20][N:19]([C@@H:22]3[O:26][C@H:25]([CH2:27][O:28][P:29]([O:32][P:33]([O:36][CH2:37][C@H:38]4[O:42][C@@H:41]([N:43]5[CH:48]=[C:47]([C:49]([NH2:51])=[O:50])[CH2:46][CH:45]=[CH:44]5)[C@H:40]([OH:52])[C@@H:39]4[OH:53])([OH:35])=[O:34])([OH:31])=[O:30])[C@@H:24]([OH:54])[C@H:23]3[O:55][P:56]([OH:59])([OH:58])=[O:57])[C:17]=2[N:18]=1.[N:5]1[C:4]([C:10]([O-:12])=[O:11])=[CH:3][CH:2]=[CH:1][C:6]=1[C:7]([O-:9])=[O:8].[CH2:1]1[CH:6]([C:7]([OH:9])=[O:8])[N:5]=[C:4]([C:10]([OH:12])=[O:11])[CH:3]=[CH:2]1

Participants

5

Reactant 2

HCID 172,3-Dihydro-2,6-pyridinedicarboxylic acid2×HCID 5884NADPH

Product 3

HCID 172,3-Dihydro-2,6-pyridinedicarboxylic acidHCID 5884NADPHHCID 5044011HCID 5044011, compound

Procedure

Dihydrodipicolinate reductase ternary crystals can be produced by mixing dihydrodipicolinate reductase with a 1.5 molar excess of NADPH and a 2.5 molar excess of 2,6-pyridinedicarboxylate (PDC) for 30-45 minutes at 4° C. to obtain a ternary complex of NADPH, PDC and dihydrodipicolinate reductase. Next, a precipitant solution containing polyethylene glycol, a salt and a buffer is provided. A droplet of dihydrodipicolinate reductase solution is mixed with a droplet of precipitant solution to obtain a mixed droplet solution. The mixed droplet solution is suspended over a well of precipitant solution in a sealed container. The vapor pressure of the precipitant solution in the well must be lower than the vapor pressure of the mixed droplet solution. The mixed droplet solution is allowed to stand for a period of time sufficient for dihydrodipicolinate reductase ternary crystals to grow to a predetermined size. Crystals appear in one to three weeks.

Workup

  1. customcan be produced

Sources & evidence

ORD record
ord-9f3d0e9b6f1948cfbc2e6b3a241cc485
Source dataset
uspto-grants-1996_09
Patent number
US05554522

Reaction notes

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