Glycolic Acid → Formate

Participants

Reaction Steps

The procedure described in Example 1 was repeated using an aqueous solution containing glycolic acid (0.500M), AMPA (0.500M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.100M), spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (14,000 IU/mL) at pH 8.5 and at 5° C. After 21 h, the HPLC yields of glyoxylate, formate, and oxalate were 85.2%, 1.5%, and 3.3%, respectively, and 5.5% glycolate remained. The remaining activities of glycolate oxidase and catalase were 49% and 93%, respectively, of their initial values.

Provenance & Evidence